Events Calendar

Join us to learn about and engage with an array of campus research service providers this summer. While you are thinking about writing articles and grant proposals or thinking up new programs, it's also a good time to refresh our memories about campus services that may enhance those research and scholarly activities.

The following will be formatted as one-hour Zoom session that will feature ten-minute service overviews by three research core facilities followed by a thirty-minute Q&A breakout sessions with each unit.

Wednesday, July 29 @ 10-11am

Metabolic Phenotyping Core:

The Metabolic Phenotyping Core provides investigators specialized and non-invasive metabolic assays that are essential in phenotyping mouse and other animal models with diabetes, its complications, obesity, and related metabolic disorders. The central services of the core include: 1) Determination of whole animal energy expenditure using Metabolic Chambers: Promethion (Sable Systems International) and CLAMS (Comprehensive Lab Animal Monitoring System, Columbus Instruments). This is a non-invasive measurement of food intake, energy expenditure, respiratory exchange ratio and physical activity. 2) Measurement of whole body composition with a Bruker MiniSpec in mice and rats. 3) Hyperinsulinemic-euglycemic clamp experiments to assess in vivo insulin action, insulin signaling, and glucose metabolism in awake mice. 4)Hyperglycemic clamp experiments to assess in vivo pancreatic b-cell function (i.e., glucose-induced insulin secretion) and the effect of hyperglycemia on glucose metabolism (i.e., glucose toxicity). 5)Mitochondrial bioenergetics: tissue/cellular/isolated mitochondria oxygen consumption using the XF-24 Extracellular Flux Analyzer; mitochondrial respirometry for tissue (permeabilized mouse heart and soleus) and isolated mitochondria with the O2K from OROBOROS. 6) Glucose and insulin tolerance tests. Specific equipment available at the Metabolic Phenotyping Core includes: a Seahorse XFe-24 analyzer, O2K from OROBOROS, two Bruker Minispecs, and Metabolic Chambers.
Presenters: Jamie Soto; Technical Director and Wojciech Grzesik; Clamps Expert

CCOM Nuclear Magnetic Resonance Facility:

The CCOM NMR Facility supports the biomedical research community with three high field (500 MHz, 600 MHz and 800 MHz) NMR spectrometers capable of probing the structure and dynamics of biomolecules using a wide range of techniques. Full spectroscopic and interpretive services are offered, as well as assistance and training for researchers who wish to perform their own experiments. Example NMR spectroscopy projects include: 1) Determination of three-dimensional structures for proteins in solution; 2) Observation of the fate of a chemical species as they are processed by a cell culture; 3) Identification of and quantification of components in a mixture, and 4) Characterization of ligand binding and aggregation in a solution.
Presenters: Liping Yu; Director and Chris Ptak; Core Facilities Research Specialist

UI High Throughput Screening Facility:

UIHTS is a high throughput platform that integrates robotics, detection systems, chemical/biologics libraries, and data/image analysis. UIHTS enables highly flexible and scalable screening approaches, not only to foster hit and lead generation for drug discovery and development through screening of large chemical/biologics libraries; but also to facilitate molecular probe discovery for mechanism of action (MOA) studies of chemical biology through screening of focused intellectually-designed compound/gRNA collections.
Presenter: Meng Wu; Director

Register for the July 29 session here: https://www.eventbrite.com/e/research-services-spotlight-series-tickets-111678675926