Next Generation Sequencing Interest Group - Targeted in-situ genome-wide mapping of histone modifications and transcription factors using CUT&RUN analysis
May 15, 2019
02:00 PM - 03:00 PM
431 Newton Road, Iowa City, IA 52246
Cleavage Under Targets and Release Using Nuclease Sequencing (CUT&RUN-sequencing) is a powerful technique used to profile histone modifications and transcription factors genome wide. CUT&RUN incorporates a novel hybrid Protein A/G-Micrococcal Nuclease (pA/G-MNase) to target antibody-protein complexes within unfixed permeabilized cells. Once activated, pA/G-MNase cleaves the adjacent DNA resulting in the targeted release of protein-DNA complexes into the supernatant for paired-end sequencing. CUT&RUN requires low cell numbers (1-100+ cells) and outperforms ChIP-Seq protocols in resolution, signal-to-noise ratio and depth of sequencing required.
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